|
KMID : 0861020080230040045
|
|
Korea Journal of Herbology
2008 Volume.23 No. 4 p.45 ~ p.49
|
|
|
Quantitative Determination of the Marker Constituent of Dioscoreae Rhizoma by the Packaging Methods
|
|
Seo Chang-Seob
Huang Dae-Sun
Lee Jun-Kyoung
Kim Ho-Kyoung
Seo Young-Bae
Shin Hyeun-Kyoo
|
|
|
Abstract
|
|
|
Objectives: To investigate the quantitative determination of marker constituents of Dioscoreae Rhizoma by the packaging methods
Methods: HPLC for the determinations of allatoin in the Dioscoreae Rhizoma. the separation method was performed on an Luna column(, Phenomenes) using solvent water-acetonitrile(2:8, v/v%) with photo diode array detector(210nm). The flow rate was 2.0 mL/min.
Results: Retention time of allatoin in HPLC chromatogram was about 4.8 min and calibration curve showed good linearity(=0.9994) at concentrations from 50.0 to 1000.0 of allantoin. Average content of allantoin by packaging methods was . In addition. weight loss rate(%) of Dioscoreae Rhizoma according to the packaging methods was .
Conclusions: The variation on content of the marker constituent of Dioscoreae Rhizoma by the packaging methods didn’t show difference.
|
|
|
KEYWORD
|
|
|
Dioscoreae Rhizoma, allantoin, HPLC, packaging method
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|